Search research articles

Search research articles

Related Experiment Videos

Regulatory volume decrease in lamprey erythrocytes: mechanisms of K+ and Cl- loss

L V Virkki¹, M Nikinmaa

¹Department of Biosciences, FIN-00014 University of Helsinki, Finland.

The American Journal of Physiology

|March 1, 1995

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Hypoxia exposure and B-type natriuretic peptide release from Langendorff heart of rats.

Acta physiologica (Oxford, England)·2016

Same author

Expiratory and sympathetic long-term facilitation and their interactions after acute intermittent hypoxia (AIH).

Acta physiologica (Oxford, England)·2016

Same author

Species differences in the adrenergic responses of fish red cells: studies on whitefish, pikeperch, trout and carp.

Fish physiology and biochemistry·2013

Same author

What is hypoxia?

Acta physiologica (Oxford, England)·2013

Same author

Control of mucus secretion in airway inflammation - what is required to infer functions?

Acta physiologica (Oxford, England)·2013

Same author

Resistance to oxidative damage but not immunosuppression by organic tin compounds in natural populations of Daubenton's bats (Myotis daubentonii).

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP·2013

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Lamprey red blood cell swelling activates conductive K+ and Cl- transport pathways. These ion channels facilitate ion and water movement, crucial for cell volume regulation in changing osmotic conditions.

Area of Science:

Physiology
Cell Biology
Ion Transport

Background:

Erythrocytes play a vital role in maintaining osmotic balance.
Understanding ion transport mechanisms is key to comprehending cell volume regulation.

Purpose of the Study:

To investigate the characteristics of swelling-activated potassium (K+) and chloride (Cl-) transport in lamprey erythrocytes.
To elucidate the conductive nature of these ion pathways.

Main Methods:

Studied unidirectional K+ and Cl- effluxes in lamprey erythrocytes under varying osmolarity.
Utilized specific inhibitors like DIOA, furosemide, and DIDS to probe transport mechanisms.
Assessed the impact of cell swelling on ion transport pathways, including Ba2+-sensitive and insensitive routes.

Related Experiment Videos

Main Results:

Swelling-activated Cl- transport was observed and inhibited by DIOA, furosemide, and DIDS.
Moderate cell swelling did not increase ouabain-insensitive K+ efflux but reduced Ba2+ inhibition, suggesting activation of a Ba2+-insensitive pathway.
DIOA did not affect K+ efflux, indicating distinct pathways for K+ and Cl-.

Conclusions:

Swelling-activated K+ and Cl- transport in lamprey erythrocytes are primarily mediated by conductive pathways.
These conductive pathways are essential for regulating cell volume in response to osmotic stress.