The influence of 2-chlorodeoxyadenosine alone and in combination with cyclophosphamide or methotrexate on normal...

The influence of 2-chlorodeoxyadenosine (2-CDA) administered alone or in combination with cyclophosphamide (CY) or methotrexate (MTX) on normal hematopoiesis in mice was investigated. The peripheral blood counts such as the number of leukocytes, platelets, and erythrocytes, hematocrit values and hemoglobin concentration were measured. The number of mononuclear cells and multipotential stem cells (CFU-S) in bone marrow was also checked. 2-CDA caused both the decrease in white cell count (granulocytes, monocytes, lymphocytes) and the suppression of mononuclear cells and multipotential stem cells in the bone marrow. The addition of CY significantly enhanced the suppression of these parameters. In contrast to CY, MTX did not augment myelotoxic effect of 2-CDA. 2-Chlorodeoxyadenosine administered singly and in combination with CY or MTX had no influence on the number of erythrocytes, hemoglobin concentration, hematocrit values and number of platelets.