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Cloning and expression of a glutamate transporter from mouse brain
1Laboratory for Neural Networks, Institute of Physical and Chemical Research, RIKEN, Saitama, Japan.
Neuroscience Letters
|September 3, 1993
Abstract:
A complementary DNA clone encoding a transporter for glutamate has been isolated from mouse brain, and its functional properties have been examined in Xenopus oocytes. The transporter displays high affinity for glutamate (Km = 72 microM) and is dependent on external Na+. The cDNA sequence predicts a protein of 543 amino acids with 6 potential transmembrane domains. Northern blot analysis indicates the mRNA encoding the glutamate transporter is expressed both in the brain and in peripheral tissues.