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Related Experiment Videos

Proliferative activity in the malignant cellular blue nevus

A Pich1, L Chiusa, E Margaria

  • 1Department of Biomedical Sciences and Human Oncology, University of Turin, Torino, Italy.

Human Pathology
|December 1, 1993
PubMed
Summary

Proliferative activity markers, argyrophilic nucleolar organizer regions (AgNORs) and proliferating cell nuclear antigen (PCNA), aid in diagnosing malignant cellular blue nevi (MCBN). These markers show significant differences compared to benign nevi, aiding diagnostic accuracy.

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Area of Science:

  • Dermatopathology
  • Oncology
  • Cell Biology

Background:

  • Malignant cellular blue nevi (MCBN) require accurate diagnostic parameters.
  • Assessing cellular proliferation is crucial for differentiating benign from malignant lesions.
  • Established markers like argyrophilic nucleolar organizer regions (AgNORs) and proliferating cell nuclear antigen (PCNA) are used in tumor diagnostics.

Purpose of the Study:

  • To evaluate the diagnostic utility of AgNOR staining and PCNA immunostaining in MCBN.
  • To compare proliferative activity markers between MCBN, benign nevi, and conventional melanomas.

Main Methods:

  • Analysis of routinely fixed, paraffin-embedded MCBN samples.
  • Application of AgNOR staining and PCNA (PC10) immunohistochemistry.

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  • DNA flow cytometry was performed.
  • Comparative analysis with cellular blue nevi (CBN), melanocytic nevi (MN), common blue nevi (BN), and malignant melanomas (MMs).

    • Main Results:

    • MCBN exhibited significantly higher AgNOR counts (8.33) and PCNA scores (31.93%) compared to CBN, MN, and BN.
    • AgNOR counts and PCNA scores in MCBN were not significantly different from conventional MMs.
    • A strong linear correlation (r = .94) was observed between AgNOR counts and PCNA scores.

    Conclusions:

    • AgNOR analysis and PCNA immunostaining are valuable adjuncts for diagnosing MCBN.
    • These proliferation markers effectively distinguish MCBN from benign melanocytic lesions.
    • The findings support the use of these markers in improving diagnostic accuracy for MCBN.