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Direct demonstration that ATP is in contact with Cys-137 in chaperonin GroEL
E S Bochkareva1, A Horovitz, A S Girshovich
1Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel.
The Journal of Biological Chemistry
|January 7, 1994
Abstract:
The nonhydrolyzable ATP analogue ATP gamma S (adenosine 5'-3-O-(thio)triphosphate) is affinity cross-linked to GroEL by formation of a disulfide bridge in a peroxide-promoted reaction. By replacing with serine each of 3 cysteine residues in GroEL, it is shown that ATP gamma S specifically cross-links to Cys-137. It is thus demonstrated that the ATP bound to GroEL is in direct contact with Cys-137.