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Differentiation of vaccine and wild-type polioviruses using polymerase chain reaction and restriction enzyme analysis
B Schweiger1, E Schreier, B Böthig
1Robert Koch Institute of the Federal Health Office, Berlin, Federal Republic of Germany.
Archives of Virology
|January 1, 1994
Abstract:
Genomic amplification followed by selective digestion of restriction enzymes was used to differentiate polioviruses. The method was based on conserved and variable components of the 5'-noncoding region. The differences between Sabin vaccine and wild-type viruses made it possible to identify rapidly an isolated poliovirus as vaccine-related or wild-type virus. A total of 60 isolates and strains were tested and all of them were correctly identified. This method is recommended as a sensitive, specific and rapid way to differentiate polioviruses in clinical isolates and environmental samples.