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A rapid manual method for CD4+ T-cell quantitation for use in developing countries

A Landay1, J L Ho, D Hom

  • 1Department of Immunology/Microbiology, Rush-Presbyterian-St Luke's Medical Center, Chicago, Illinois 60612.

AIDS (London, England)
|December 1, 1993
PubMed
Summary

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This summary is machine-generated.

A manual Cytosphere assay accurately quantifies CD4+ T-cells, showing high correlation with flow cytometry. This method is suitable for resource-limited settings, aiding HIV monitoring.

Area of Science:

  • Immunology
  • Clinical Diagnostics
  • Public Health

Background:

  • Accurate CD4+ T-cell counts are crucial for monitoring HIV-1 infection and guiding treatment.
  • Standard flow cytometry methods require specialized equipment and trained personnel, limiting accessibility in resource-limited settings.

Purpose of the Study:

  • To evaluate the accuracy and reliability of the manual Cytosphere assay for quantifying absolute CD4+ T-cell numbers.
  • To assess the potential of the Cytosphere assay as an alternative to flow cytometry in diverse laboratory settings.

Main Methods:

  • A cross-sectional study compared the manual Cytosphere assay with standardized flow cytometry for absolute CD4+ T-cell counts.
  • Blood specimens from 382 individuals (294 HIV-1-seropositive, 88 HIV-1-seronegative) were analyzed.
Keywords:
Acquired Immunodeficiency SyndromeAmericasAntibodiesBiologyComparative StudiesCross Sectional AnalysisCytologyDeveloped CountriesDeveloping CountriesDiseasesExaminations And DiagnosesHematologic TestsHiv InfectionsImmunityImmunologic FactorsLaboratory Examinations And DiagnosesLaboratory ProceduresMethodological StudiesNorth AmericaNorthern AmericaPhysiologyResearch MethodologyStudiesUnited StatesViral Diseases

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  • The study was conducted across university research hospitals in the United States and Africa.

    • Main Results:

    • The Cytosphere assay demonstrated a strong overall correlation (0.912) with flow cytometry for CD4+ T-cell quantification.
    • It showed high predictive values: 96% for identifying counts > 200 x 10(6)/l and 92% for counts < 200 x 10(6)/l.
    • Results were consistent across samples from both the United States and Africa.

    Conclusions:

    • The manual Cytosphere assay is a reliable method for quantifying CD4+ T-cells.
    • Its strong correlation with flow cytometry suggests its utility in settings with limited laboratory infrastructure.
    • This assay holds potential for improving HIV patient management in developing countries.