Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Potassium channels in colonic crypts

G I Sandle1, C M McNicholas, R B Lomax

  • 1Department of Medicine, Hope Hospital (University of Manchester, School of Medicine, Salford, UK.

Lancet (London, England)
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rapid stimulation of human renal ENaC by cAMP in Xenopus laevis oocytes.

Journal of physiology and biochemistry·2012
Same author

Basolateral potassium (IKCa) channel inhibition prevents increased colonic permeability induced by chemical hypoxia.

American journal of physiology. Gastrointestinal and liver physiology·2010
Same author

Apical potassium (BK) channels and enhanced potassium secretion in human colon.

QJM : monthly journal of the Association of Physicians·2009
Same author

Regulation of colonic apical potassium (BK) channels by cAMP and somatostatin.

American journal of physiology. Gastrointestinal and liver physiology·2009
Same author

Altered cryptal expression of luminal potassium (BK) channels in ulcerative colitis.

The Journal of pathology·2007
Same author

Enhanced large intestinal potassium permeability in end-stage renal disease.

The Journal of pathology·2005

Researchers identified specific potassium channels in human colon crypt cells. These channels are crucial for chloride secretion and may offer a target for treating secretory diarrhea.

Area of Science:

  • Gastroenterology
  • Cell Physiology
  • Ion Channel Biology

Background:

  • Intestinal epithelia secrete chloride (Cl-), a key process in secretory diarrhea, primarily occurring in intestinal crypts.
  • Basolateral membrane potassium (K+) channel activity is vital for Cl- secretion, maintaining cellular hyperpolarization and driving Cl- efflux.
  • Understanding these K+ channels is crucial for developing treatments for diarrheal diseases.

Purpose of the Study:

  • To characterize the properties of K+ channels in the basolateral membrane of human colonic crypt cells.
  • To investigate the regulation of these K+ channels by intracellular messengers.
  • To explore the potential of these K+ channels as a therapeutic target for secretory diarrhea.

Main Methods:

  • Utilized patch-clamp techniques to study ion channel activity.

Related Experiment Videos

  • Investigated the effects of voltage, calcium (Ca2+), and barium (Ba2+) on channel function.
  • Examined the role of cyclic adenosine monophosphate (cAMP) and Ca2+ in regulating channel activity.

    • Main Results:

    • Identified low conductance K+ channels in the basolateral membrane of human colonic crypt cells.
    • These K+ channels are sensitive to voltage, Ca2+, and blocked by Ba2+.
    • Cytosolic cAMP and Ca2+ regulate these K+ channels, mirroring their known roles in stimulating Cl- secretion.

    Conclusions:

    • The identified K+ channels are a significant component of the basolateral membrane in human intestinal crypt cells.
    • These channels are regulated by key intracellular signaling molecules involved in chloride secretion.
    • This specific population of K+ channels represents a potential pharmacological target for managing secretory diarrhea.