Physicochemical properties of four tymoviruses

Isoelectric point (pI), free mobility and retardation coefficient (KR) of turnip yellow mosaic virus (TYMV), erysimum latent virus (ELV), belladonna mottle virus (BelMV) and scrophullaria mottle virus (ScrMV) have been studied. The use of Ferguson plots for the physical identification of these four viruses and for confirmation of their identity is discussed. For TYMV, ELV, BelMV and ScrMV the pI values of 3.64, 4.73, 6.25 and 3.95, respectively, were found. Using KR the differences between the diameter of particles of the four tymoviruses were estimated. The particle diameter decreases as follows: ELV, ScrMV, TYMV, BelMV.