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Related Experiment Videos

The elusive peripheral blood hemopoietic stem cell

D E Hogge1, H J Sutherland, P M Lansdorp

  • 1Terry Fox Laboratory, B.C. Cancer Research Centre, Vancouver General Hospital, Canada.

Seminars in Hematology
|October 1, 1993
PubMed
Summary

Primitive hemopoietic stem cells, known as long-term culture initiating cells (LTC-IC), circulate in peripheral blood. These cells can effectively support hematopoietic reconstitution after aggressive cancer therapies.

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Area of Science:

  • Hematology
  • Stem Cell Biology
  • Cancer Therapy

Background:

  • Long-term culture initiating cells (LTC-IC) are primitive hematopoietic progenitors.
  • LTC-IC exhibit characteristics of marrow-repopulating stem cells, including high proliferative and multilineage-differentiative capacity.
  • LTC-IC are resistant to 4-hydroperoxy-cyclophosphamide (4-HC) and can be mobilized into peripheral blood.

Purpose of the Study:

  • To investigate the presence and function of primitive hematopoietic cells in human peripheral blood.
  • To assess the potential of mobilized peripheral blood stem cells for hematopoietic reconstitution.

Main Methods:

  • Culture of primitive hematopoietic progenitors (LTC-IC) on supportive mesenchymal feeder layers.
  • Mobilization of peripheral blood stem cells using cytotoxic chemotherapy and/or growth factors.

Related Experiment Videos

  • Evaluation of engraftment kinetics following high-dose chemo/radiotherapy using mobilized peripheral blood cells.

    • Main Results:

    • LTC-IC demonstrate characteristics of stem cells, including multilineage differentiation and resistance to 4-HC.
    • Primitive hematopoietic cells, including LTC-IC and clonogenic precursors, circulate in normal peripheral blood.
    • Mobilized peripheral blood stem cells facilitate more rapid engraftment compared to autologous marrow in high-dose therapy.

    Conclusions:

    • Primitive hematopoietic cells circulate in human peripheral blood.
    • Mobilized peripheral blood stem cells can effectively support hematopoietic reconstitution after aggressive therapy.
    • LTC-IC represent a viable source for autologous stem cell transplantation in cancer treatment.