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Related Experiment Videos

Differences in outer membrane characteristics between gallstone-associated bacteria and normal bacterial flora

L A Wetter1, R M Hamadeh, J M Griffiss

  • 1Department of Surgery, University of California, San Francisco.

Lancet (London, England)
|February 19, 1994
PubMed
Summary

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Bacteria found in pigment gallstones possess unique membrane characteristics, including P1 fimbriae and alpha-galactosyl residues. These traits may enable bacteria to colonize the biliary tree and form gallstones.

Area of Science:

  • Microbiology
  • Gastroenterology
  • Pathogenesis of Gallstones

Background:

  • Previous scanning electron microscopy (SEM) studies suggested bacteria presence in pigment gallstones.
  • The specific characteristics of these bacteria and their role in gallstone formation remain unclear.

Purpose of the Study:

  • To culture bacteria from pigment gallstones.
  • To characterize the membrane properties of these bacteria.
  • To investigate the association between bacteria in gallstones and clinical conditions.

Main Methods:

  • Gallstones from 54 patients were analyzed using SEM.
  • Bacteria were cultured from stones where SEM detected them.
  • Bacterial membrane characteristics, including fimbriae and erythrocyte agglutination, were studied.

Related Experiment Videos

  • Antibody binding assays were performed.

    • Main Results:

    • SEM detected bacteria in brown pigment stones, some black pigment stones, and mixed stones, but not pure cholesterol stones.
    • 20 bacterial species were cultured from positive gallstones.
    • Bacteria-containing gallstones were linked to clinical sepsis and cholangitis.
    • All cultured bacteria exhibited P1-specific fimbriae and bound anti-Gal (alpha-galactosyl residues); some had Forssman-antigen-specific fimbriae, but none had mannose-specific fimbriae.

    Conclusions:

    • Bacteria are frequently present in pigment gallstones and can be cultured.
    • The unique membrane features (P1 fimbriae, anti-Gal binding) differentiate these bacteria from typical gut flora.
    • These properties may facilitate bacterial colonization of the biliary tree, contributing to pigment gallstone formation.