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Related Experiment Videos

Molecular marker linkage map for apple

M Hemmat1, N F Weeden, A G Manganaris

  • 1Department of Horticultural Sciences, Cornell University, Geneva, New York 14456.

The Journal of Heredity
|January 1, 1994
PubMed
Summary

Researchers developed detailed genetic linkage maps for two apple (Malus domestica) clones, White Angel and Rome Beauty. This work expands the standard apple map to approximately 360 markers, aiding future genetic studies.

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Area of Science:

  • Horticulture
  • Plant Genetics
  • Genomics

Background:

  • Developing high-density genetic linkage maps is crucial for understanding plant genomes.
  • Apple (Malus domestica) genetics research benefits from comprehensive mapping resources.

Purpose of the Study:

  • To construct and integrate genetic linkage maps for two distinct apple clones, White Angel and Rome Beauty.
  • To expand the existing standard apple genetic map with new markers and linkage information.

Main Methods:

  • Utilized isozyme and DNA polymorphisms in a population derived from a Rome Beauty x White Angel cross.
  • Employed a double pseudotestcross mapping strategy to analyze segregation data.
  • Integrated marker data to construct linkage groups and estimate genetic distances (centimorgans, cM).

Main Results:

  • Generated linkage maps for White Angel (253 markers, 24 groups, 950 cM) and Rome Beauty (156 markers, 21 groups).
  • Identified homologous linkage groups between the two apple clones, integrating them into a standard map of ~360 markers.
  • Achieved high marker density (10-15 cM) in most linkage groups.
  • Found no evidence of sex-specific differences in recombination rates in comparable genomic regions.

Conclusions:

  • The integrated apple linkage map provides a robust framework for quantitative trait loci (QTL) analysis and marker-assisted selection.
  • The study enhances the genetic understanding of apple and facilitates genome-wide association studies.
  • The findings contribute to the development of improved apple cultivars through advanced breeding strategies.

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