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[Detection of translocation (15;17) using cytogenetic and molecular techniques. Comparative study in 15 patients with
E Arranz1, J Benítez, A Santón
1Departamento de Genética y Hematología, Fundación Jiménez Díaz, Madrid.
Purposes:
To analyze the reproducibility to detect the t (15; 17) with molecular and cytogenetic techniques.
Patients And Methods:
Fifteen cases of acute promyelocytic leukaemia were studied by means of cytogenetic techniques (GTG banding) and molecular ones (K/S probe and HindIII, EcoRI and BamHI restriction enzymes).
Results:
The t (15; 17) was detected with cytogenetic techniques in 60% cases and with molecular ones in 33% cases. Using both (cytogenetic and molecular) techniques the translocation was detected in 73% cases.
Conclusions:
The use of both techniques may be very useful in order to get a better diagnostic and a closer control of the patient's clinical evolution.