Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Murine heparin cofactor II: purification, cDNA sequence, expression, and gene structure

G S Zhang1, J H Mehringer, V M Van Deerlin

  • 1Division of Hematology-Oncology, Washington University School of Medicine, St Louis, Missouri 63110.

Biochemistry
|March 29, 1994
PubMed
Summary

Murine plasma contains two forms of Heparin Cofactor II (HCII) with identical amino acid sequences but different N-linked oligosaccharides. This suggests HCII is highly conserved between humans and mice.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Distinguish immunoglobulin heavy chain variable region gene usage and mutational status of the leukemic B cells in Chinese patients with chronic lymphocytic leukemia].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2022
Same author

[Preliminary efficacy and safety analysis of carrelizumab combined with apatinib in patients with middle-advanced liver cancer progressed after drug-eluting beads-transcatheter arterial chemoembolization].

Zhonghua yi xue za zhi·2021
Same author

The persistent impacts of polyester microfibers on soil bio-physical properties following thermal treatment.

Journal of hazardous materials·2021
Same author

[Transcriptome sequencing-based classification and quantification of IKZF1 transcript isoforms in B-cell acute lymphoblastic leukemia].

Zhonghua yi xue za zhi·2021
Same author

Polyester microfiber and natural organic matter impact microbial communities, carbon-degraded enzymes, and carbon accumulation in a clayey soil.

Journal of hazardous materials·2020
Same author

Variations in aggregate-associated organic carbon and polyester microfibers resulting from polyester microfibers addition in a clayey soil.

Environmental pollution (Barking, Essex : 1987)·2019

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Heparin Cofactor II (HCII) is a plasma glycoprotein that inhibits thrombin.
  • Human HCII is a single protein, but murine plasma exhibits HCII activity in two distinct proteins.

Purpose of the Study:

  • To investigate the nature of the two HCII proteins found in murine plasma.
  • To characterize the gene and mRNA for murine HCII.
  • To compare the conservation of HCII between humans and mice.

Main Methods:

  • Protein purification and N-terminal sequencing.
  • Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase treatment.
  • cDNA cloning and sequencing.
  • Northern and Southern blot analyses.

Related Experiment Videos

  • Gene mapping using restriction fragment length polymorphisms.

    • Main Results:

    • Murine plasma contains two HCII forms (68 and 72 kDa) with identical N-terminal sequences and reactivity to anti-HCII antibodies.
    • Deglycosylation yields a single 54-kDa band, indicating differences in N-linked oligosaccharides.
    • Murine HCII cDNA sequence shows 87% identity to human HCII.
    • A single murine HCII gene (Hcf2) maps to chromosome 16 and shares intron/exon organization with the human gene.

    Conclusions:

    • Murine plasma contains two HCII isoforms differing in glycosylation, not amino acid sequence.
    • The murine HCII gene structure is highly conserved compared to the human gene.
    • Heparin Cofactor II is more conserved between humans and mice than other serpins.