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Related Experiment Videos

Intercellular adhesion molecule-1 expression by bladder cancer cells: functional effects

S C Campbell1, K Tanabe, J P Alexander

  • 1Department of Urology, Cleveland Clinic Foundation, Ohio 44195.

The Journal of Urology
|May 1, 1994
PubMed
Summary
This summary is machine-generated.

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Intercellular adhesion molecule-1 (ICAM-1) aids lymphokine activated killer (LAK) cell binding to bladder cancer cells, especially after cytokine treatment. However, ICAM-1 plays a minor role in LAK cell-mediated cancer cell killing.

Area of Science:

  • Immunology
  • Oncology
  • Cell Biology

Background:

  • Intravesical BCG therapy for bladder cancer involves cytokines that may modulate immune cell interactions.
  • Intercellular adhesion molecule-1 (ICAM-1) and leukocyte function-associated antigen-1 (LFA-1) are key adhesion molecules in immune responses.
  • Understanding ICAM-1 and LFA-1 roles in bladder cancer and lymphokine-activated killer (LAK) cell interactions is crucial for immunotherapy.

Purpose of the Study:

  • To investigate the role of ICAM-1 and LFA-1 in bladder cancer cell and LAK cell interactions.
  • To assess ICAM-1 expression and modulation by cytokines in bladder cancer cell lines.
  • To determine the impact of ICAM-1 and LFA-1 on LAK cell adherence and cytolysis of bladder cancer cells.

Main Methods:

  • Immunocytometry and Northern blot analysis to assess ICAM-1 expression.

Related Experiment Videos

  • Cytokine (IFN-gamma, TNF-alpha) treatment of bladder cancer cell lines.
  • Blocking assays using monoclonal antibodies against ICAM-1 and LFA-1 to study LAK cell adherence and cytolysis.

    • Main Results:

    • Four of five bladder cancer cell lines constitutively expressed ICAM-1, upregulated by IFN-gamma and TNF-alpha.
    • Anti-ICAM-1 and anti-LFA-1 antibodies significantly reduced LAK cell adherence to ICAM-1-expressing cells.
    • Anti-LFA-1 antibodies reduced LAK cell-mediated cytolysis, while anti-ICAM-1 antibodies had minimal effect.

    Conclusions:

    • ICAM-1 is important for LAK cell adherence to bladder cancer cells, with enhanced binding after cytokine stimulation.
    • LFA-1 is more critical for LAK cell-mediated cytolysis than ICAM-1.
    • Adhesion molecules like ICAM-1 may mediate immune responses in bladder cancer following intravesical BCG therapy.