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A simple method for maintaining fastidious organisms

C H Park

    American Journal of Clinical Pathology
    |November 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Preserving nutritionally demanding organisms and anaerobes at -50°C in glycerol broth maintains viability for over two years. This cost-effective method simplifies long-term microbial sample storage.

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    Area of Science:

    • Microbiology
    • Cryopreservation
    • Anaerobic microbiology

    Background:

    • Maintaining viability of fastidious organisms and anaerobes is crucial for research.
    • Current preservation methods may be costly or inconvenient.
    • Long-term storage solutions are needed for microbial cultures.

    Purpose of the Study:

    • To evaluate the efficacy of glycerol broth for long-term preservation of nutritionally fastidious organisms and anaerobes.
    • To determine the viability of microbial strains after storage at -50°C.
    • To assess the convenience and cost-effectiveness of this preservation method.

    Main Methods:

    • Nutritionally fastidious organisms and anaerobes were cultured.
    • Cultures were suspended in glycerol broth.

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  • Samples were stored at -50°C for a minimum of two years.
  • Viability assessments were performed post-storage.

    • Main Results:

    • Microbial strains, including fastidious organisms and anaerobes, maintained viability for at least two years when stored in glycerol broth at -50°C.
    • No loss of viability was observed in the tested strains.
    • The method proved to be convenient for sample handling and storage.

    Conclusions:

    • Glycerol broth is an effective and reliable medium for the long-term cryopreservation of nutritionally fastidious organisms and anaerobes at -50°C.
    • This preservation technique offers a convenient and cost-effective alternative for microbial sample management.
    • The findings support the widespread adoption of this method in microbiology laboratories.