Related Experiment Videos
Experimental hypersensitivity pneumonitis. Effect of CD4 cell depletion
M Schuyler1, K Gott, B Edwards
1Department of Medicine, Albuquerque Veterans Administration Medical Center, NM 87108.
Summary
CD4+ T-cells are essential for adoptive experimental hypersensitivity pneumonitis (EHP) development. However, the acute lung inflammation caused by Micropolyspora faeni does not require CD4+ T-cells.
Area of Science:
- Immunology
- Pulmonary Medicine
- Cellular Biology
Background:
- Experimental hypersensitivity pneumonitis (EHP) is a lung inflammation model.
- Previous studies identified Thy1.2+, CD4+, Ia-T cells in adoptive EHP transfer.
Purpose of the Study:
- To identify cells responsible for pulmonary inflammation in recipient animals.
- To investigate the role of CD4+ cells in adoptive EHP and direct EHP.
Main Methods:
- CD4+ cell depletion in recipient mice using monoclonal antibody GK1.5.
- Administration of Micropolyspora faeni-sensitized spleen cells (SC) and intratracheal (i.t.) challenge.
- Assessment of pulmonary inflammatory changes in adoptive and direct EHP models.
Main Results:
- Depletion of CD4+ cells prevented the transfer of EHP.
- Adoptive EHP transfer was successful in naive or irrelevant antibody-treated animals.
- Direct EHP induced neutrophilic lung infiltration, unaffected by CD4+ cell depletion.
Conclusions:
- Adoptive EHP development is dependent on the presence of CD4+ cells.
- The acute inflammatory response to M. faeni in the lung is CD4+ cell-independent.