[Analysis of mdr-1 mRNA and P 170 glycoprotein in cytotoxic resistant sublines]

Multidrug resistant sublines KBV200 and HCTV2000 were established by continuous selection from human KB and HCT cell lines cultured in medium in the presence of vincristine. The mdr-1 mRNA in total RNA from the two sublines was detected by dot blotting with HRP-labelled mdr-1 cDNA probe and enhanced chemiluminescent autography and the P170 glycoprotein in their cells was detected by immunohistochemistry. The mdr-1 gene expression level of KBV200 and HCTV2000 was higher than their wild counterparts. It was shown that the drug resistant properties of the two sublines were related to mdr-1 gene expression. The non-radioactive technique for analysis of specific mRNA and protein could be generalized for clinical research of gene expression.