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Enhancement of CDP-DG:inositol transferase activity in src- and erbB2-transformed cells
M Imoto1, Y Taniguchi, H Fujiwara
1Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan.
Experimental Cell Research
|May 1, 1994
Abstract:
Phosphatidylinositol (PI) synthesis was activated in Rous sarcoma virus-infected NIH3T3 or activated erbB2-transformed NIH3T3 cells. The in vitro activity of CDP-DG:inositol transferase prepared from these cells was also higher than that from normal parent NIH3T3 cells, although phospholipase C and PI kinase activities were not significantly different among these cells. A tyrosine kinase inhibitor, erbstatin, inhibited the PI synthesis in cultured cells, suggesting that Src and ErbB2-associated tyrosine kinases are involved in activation of CDP-DG:inositol transferase in these cell lines.