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Paired box gene expression in Wilms' tumor
1Department of Surgery, Medical University of South Carolina, Charleston 29425.
Journal of Pediatric Surgery
|February 1, 1994
Summary
Paired box (PAX) genes and WT1 are frequently expressed in Wilms
Area of Science:
- Pediatric Oncology
- Molecular Biology
- Genetics
Background:
- Wilms' tumor (WT) is a pediatric kidney cancer resembling fetal development.
- The paired box (PAX) gene family is implicated in WT development.
Purpose of the Study:
- To investigate the expression of PAX2, PAX8, and WT1 genes in Wilms' tumors.
- To compare gene expression patterns between primary and heterotransplanted WTs and other childhood tumors.
Main Methods:
- Northern blot analysis was used to detect gene expression.
- RNA was isolated from primary WTs, heterotransplanted WTs, and other embryonal tumors.
- Hybridization was performed using probes for PAX2, PAX8, and WT1.
Main Results:
- PAX2 and WT1 were expressed in all primary WTs; PAX8 was present in most.
- PAX2, PAX8, and WT1 were concurrently expressed in a majority of heterotransplanted WTs.
- These genes were largely absent in other childhood renal and nonrenal tumors.
Conclusions:
- PAX genes and WT1 show significant expression in Wilms' tumors.
- Their specific roles in WT pathogenesis and interaction with WT1 require further investigation.
- Understanding these genes offers insights into kidney development and WT pathology.