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Related Experiment Videos

Soluble intercellular adhesion molecule-1 in arthritis

A E Koch1, M R Shah, L A Harlow

  • 1Department of Medicine, Northwestern University Medical School, Chicago, Illinois 60611.

Clinical Immunology and Immunopathology
|May 1, 1994
PubMed
Summary
This summary is machine-generated.

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Soluble intercellular adhesion molecule-1 (sICAM-1) is elevated in rheumatoid arthritis (RA) synovial fluid, correlating with leukocyte counts. Mononuclear cells in RA synovial fluid are the primary producers of sICAM-1, suggesting its role in inflammatory cell trafficking.

Area of Science:

  • Immunology
  • Rheumatology
  • Cell Biology

Background:

  • Rheumatoid arthritis (RA) is a chronic inflammatory disorder characterized by joint inflammation.
  • Soluble intercellular adhesion molecule-1 (sICAM-1) is a marker implicated in inflammatory processes.
  • Understanding the source and role of sICAM-1 in RA pathogenesis is crucial.

Purpose of the Study:

  • To investigate the presence and levels of sICAM-1 in rheumatoid arthritis (RA) and other inflammatory arthritides.
  • To identify the specific cells responsible for sICAM-1 production in the synovial environment of RA patients.
  • To explore the potential role of sICAM-1 in leukocyte trafficking in RA.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) was used to quantify sICAM-1 levels in synovial fluid and blood.

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  • Cells, including neutrophils (PMNs) and mononuclear cells, were isolated from RA synovial fluids and tissues.
  • sICAM-1 release from isolated cells was measured, with and without stimulation (phytohemagglutinin, lipopolysaccharide).

    • Main Results:

    • Synovial fluid sICAM-1 levels were significantly higher in RA and other inflammatory arthritides compared to osteoarthritis (OA).
    • sICAM-1 levels in synovial fluid showed a positive correlation with synovial fluid leukocyte counts.
    • Mononuclear cells from RA synovial fluid were the major producers of sICAM-1, while RA synovial fibroblasts produced low levels.
    • Neutrophils (PMNs) also released sICAM-1 spontaneously.

    Conclusions:

    • Elevated sICAM-1 in RA synovial fluid suggests its involvement in the inflammatory disease process.
    • Mononuclear cells are the primary source of sICAM-1 in the RA synovial compartment.
    • sICAM-1 likely plays a role in the recruitment and trafficking of inflammatory leukocytes into RA joints.