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Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
Published on: October 9, 2009
Anatomy of a DNA replication fork revealed by reconstitution of SV40 DNA replication in vitro
1Cold Spring Harbor Laboratory, New York 11724.
Abstract:
Complete enzymatic replication of DNA from the simian virus 40 origin has been reconstituted with T antigen and highly purified cellular proteins. DNA polymerase-alpha/primase functions primarily to synthesize RNA-DNA primers for initiation of DNA replication at the origin and for priming each Okazaki fragment. A polymerase switching mechanism requiring replication factor C and the proliferating cell nuclear antigen allows two molecules of DNA polymerase-delta to replicate both strands of the double helix conjointly.
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