Related Experiment Videos
Polymorphisms of apolipoprotein B gene in relation to coronary heart disease in Chinese Han nationality
Insights
Genetic variations in the apolipoprotein B (apoB) gene, specifically at the XbaI site, are linked to coronary heart disease (CHD) risk. The X+ allele was more common in CHD patients and associated with lower HDL-C levels.
Area of Science:
- Genetics
- Cardiovascular Disease
- Lipid Metabolism
Background:
- Coronary heart disease (CHD) is a leading cause of mortality worldwide.
- Apolipoprotein B (apoB) gene polymorphisms have been implicated in CHD risk.
- Understanding genetic factors influencing lipid metabolism is crucial for CHD prevention.
Purpose of the Study:
- To investigate the association between apolipoprotein B (apoB) gene polymorphisms (XbaI and EcoRI sites) and coronary heart disease (CHD) in a Chinese Han population.
- To examine the impact of these polymorphisms on lipid metabolism parameters.
Main Methods:
- Case-control study involving 80 CHD patients and 60 healthy controls from the Chinese Han population.
- Analysis of allele frequencies for XbaI and EcoRI restriction fragment length polymorphisms (RFLPs) in the apoB gene.
- Comparison of lipid and lipoprotein levels between different genotypes within the CHD patient group.
Main Results:
- The X+ allele (presence of XbaI cutting site) was significantly more frequent in CHD patients (0.10) compared to controls (0.01, P < 0.001).
- The E- allele (lack of EcoRI cutting site) showed a trend towards higher frequency in CHD patients (0.11) versus controls (0.05), but this was not statistically significant (P < 0.1).
- In CHD patients, the X+ allele was associated with lower levels of high-density lipoprotein cholesterol (HDL-C) and apolipoprotein AI (apoAI), but not other lipid parameters. EcoRI polymorphism showed no significant influence on lipid levels.
Conclusions:
- Genetic variation at the XbaI site of the apoB gene may contribute to the determination of plasma HDL-C levels.
- This variation might play a role in the development of CHD in the Chinese Han population.
- The EcoRI polymorphism of the apoB gene does not appear to significantly influence lipid levels or CHD risk in this population.
Abstract:
Two polymorphic sites of apolipoprotein B (apoB) gene, XbaI and EcoRI, were examined in 80 patients with documented coronary heart disease (CHD) and 60 healthy individuals selected from a population of Chinese Han nationality. Allele frequencies at these sites were compared between the patients and controls and their impact on lipid metabolism was also investigated. The frequency of X+ allele (presence of XbaI cutting site) in CHD patients was 0.10, which was significantly higher than 0.01 in controls (P < 0.001). The same trend was detected for frequency of E-allele (lack of EcoRI cutting site) when it was compared between CHD patients and controls (0.11 vs 0.05), the difference was not statistically significant (P < 0.1, > 0.05). There was no relationship between XbaI RFLP and EcoRI RFLP. In the CHD patients, X+ allele was associated with the lower levels of HDL-C and apoAI when compared with X- allele, while no associations were found of X+ allele with other lipid parameters. On the contrary, the EcoRI polymorphism had no significant influence on plasma lipid and lipoprotein levels. Therefore, it was suggested that genetic variation at XbaI site of apoB gene might contribute to the determination of plasma HDL-C level and development of CHD in people among Chinese Han nationality.