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A rapid and simple method for the purification of transglutaminase from Streptoverticillium mobaraense
U Gerber1, U Jucknischke, S Putzien
1Fachbereich Chemische Technologie, Fachhochschule Darmstadt, Germany.
The Biochemical Journal
|May 1, 1994
Abstract:
Transglutaminase from Streptoverticillium mobaraense was partially purified by ion-exchange chromatography on a weak acid material and hydrophobic chromatography. The separation with a strong acid ion-exchanger produces homogeneous transglutaminase, in a single step and with high yields, directly from the centrifuged and filtered culture fluid of the micro-organism. The procedure reproduced several times could be also carried out on a larger scale with the optimized parameters of the laboratory isolations. The purified enzyme demonstrated good storage stability.