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Timed-release capsule method for coliform enumeration

W W Lanz, P A Hartman

    Applied and Environmental Microbiology
    |November 1, 1976
    PubMed
    Summary
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    A novel capsule method enhances the resuscitation of heat-injured Escherichia coli in water analysis. This technique improves coliform detection by allowing injured cells to repair before inhibition, offering a combined presumptive and confirmed test.

    Area of Science:

    • Microbiology
    • Environmental Science
    • Food Science

    Background:

    • Conventional methods for detecting coliforms in water can be hindered by injured cells.
    • Heat-injured Escherichia coli may not be detected by standard selective media.

    Purpose of the Study:

    • To develop and evaluate a novel capsule-based method for improved detection of heat-injured Escherichia coli.
    • To assess the efficacy of this method in water sample analysis.

    Main Methods:

    • Wax-coated capsules containing brilliant green and oxgall were added to modified lactose broths.
    • Inhibitory ingredients diffused gradually, allowing injured cell repair.
    • Compared capsule method with conventional brilliant green bile 2% broth, violet red bile agar, most-probable-number, membrane filtration, and pour plate methods.

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    Main Results:

    • Resuscitation of heat-injured Escherichia coli B cells was observed in capsule-containing media.
    • No statistically significant differences in coliform counts were found between the capsule method and conventional methods for water samples.
    • The capsule method demonstrated potential as a combined presumptive and confirmed test for water examination.

    Conclusions:

    • The capsule method facilitates the resuscitation of heat-injured Escherichia coli, improving detection accuracy in water.
    • This method shows promise for water quality assessment, offering a dual presumptive and confirmed test.
    • Further research is needed to adapt the capsule method for food sample analysis.