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Cultured neurons infected with an HSV-1-derived vector remain electrically excitable and responsive to
R H Farkas1, S Nakajima, Y Nakajima
1Department of Anatomy and Cell Biology (M/C 512), University of Illinois, College of Medicine at Chicago 60612.
Neuroscience Letters
|January 3, 1994
Abstract:
Vectors derived from herpes simplex virus type 1 (HSV-1) have allowed foreign genes to be expressed in differentiated postmitotic neurons, but the usefulness of these infected neurons for electrophysiological studies has not been examined. Using a latent, non-pathogenic recombinant HSV-1 virus, we expressed E. coli beta-galactosidase in identified rat cholinergic and dopaminergic neurons in culture. The electrophysiological properties of the infected cholinergic neurons were examined. The neurons remained electrically excitable and responsive to neurotransmitter.