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Immunofluorescent localisation of cathepsin D in trophoblastic cells in tissue culture
Abstract:
Cathepsin D was localised by immunofluorescence within four different types of cells derived from human trophoblast in monolayer culture. Cells were stained with freshly reduced Fab' fragments of sheep anti-(human cathepsin D) antiserum followed by FITC conjugate of pig anti-(sheep Fab') Fab' fragments. The green fluorescence seen in cytoplasmic droplets were characteristic of the appearance of lysosomes in other cells.