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[The effect of L-glutamic acid on the ADP-induced aggregation and Ca, Mg-ATpase activity of human thrombocytes]
In the study of L-glutaminic acid effects on human platelet functional activity it was established that 30-60-min treatment of platelet-rich plasma with glutamate in concentration 0.1-1.0 mM at 37 degrees C results in a marked platelet activation. The latter noticeably enhances ADP-induced aggregation persistent against antiaggregation adenosine concentrations. With increasing duration of the incubation a glutaminic acid minimal effective concentration lowers. Proaggregatory and anti-adenosine effects of L-glutaminic acid are inhibited by kainic acid. The experiments evidence that 30-min preincubation of platelet membranes with 1 mM L-glutaminic acid produces inhibition of cAMP-induced activation of Ca, Mg-ATPase returning the activity of the enzyme to baseline levels. No glutamate-related changes in calcium pump of platelets are reported.
In the study of L-glutaminic acid effects on human platelet functional activity it was established that 30-60-min treatment of platelet-rich plasma with glutamate in concentration 0.1-1.0 mM at 37 degrees C results in a marked platelet activation. The latter noticeably enhances ADP-induced aggregation persistent against antiaggregation adenosine concentrations. With increasing duration of the incubation a glutaminic acid minimal effective concentration lowers. Proaggregatory and anti-adenosine effects of L-glutaminic acid are inhibited by kainic acid. The experiments evidence that 30-min preincubation of platelet membranes with 1 mM L-glutaminic acid produces inhibition of cAMP-induced activation of Ca, Mg-ATPase returning the activity of the enzyme to baseline levels. No glutamate-related changes in calcium pump of platelets are reported.