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Related Experiment Videos

Host-cell receptors for Sindbis virus

J H Strauss1, K S Wang, A L Schmaljohn

  • 1Division of Biology, California Institute of Technology, Pasadena.

Archives of Virology. Supplementum
|January 1, 1994
PubMed
Summary

Sindbis virus uses multiple host cell proteins as receptors, explaining its broad host range. These receptors, including a laminin receptor, are conserved across diverse species like insects and vertebrates.

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Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Sindbis virus (SINV) exhibits a remarkably wide host range, infecting various mosquito vectors and numerous vertebrate species.
  • Understanding the molecular mechanisms of viral entry is crucial for comprehending host tropism and viral pathogenesis.

Purpose of the Study:

  • To identify and characterize the host cell receptors utilized by Sindbis virus for cell entry.
  • To elucidate the mechanisms underlying Sindbis virus's broad host range.

Main Methods:

  • Utilized anti-idiotype antibodies against neutralizing antibodies targeting Sindbis virus glycoprotein E2 to identify chicken cell receptors.
  • Employed monoclonal antibodies to identify putative receptors in mammalian and mosquito cells, focusing on high-affinity laminin receptors.

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Main Results:

  • A 63-kDa protein was identified as a potential Sindbis virus receptor in chicken cells.
  • A 67-kDa protein, identified as a high-affinity laminin receptor, was implicated as a receptor in both mammalian and mosquito cells.

Conclusions:

  • Sindbis virus employs multiple distinct proteins as cellular receptors, contributing to its extensive host range.
  • The utilization of highly conserved proteins, such as the laminin receptor, across different animal kingdoms facilitates Sindbis virus's broad infectivity.