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Study on the surface polypeptides isolated from human lymphoblastoid ce-ls by ionic shock

E H Lo, M H Ng, W S Ng

Biochimica Et Biophysica Acta

|October 22, 1976

Summary

Ionic shock treatment efficiently extracts Raji cell surface proteins. These extracted components are recognized by antibodies, confirming their surface localization and potential for immunologic studies.

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Area of Science:

Cell biology
Immunology
Biochemistry

Background:

Raji cells are a human B lymphoblast cell line.
Understanding cell surface components is crucial for immunology and diagnostics.

Purpose of the Study:

To develop an efficient method for extracting Raji cell surface components.
To characterize the extracted polypeptides and their surface accessibility.

Main Methods:

Ionic shock treatment with 10% glycerol.
Solubilization and analysis of released polypeptides.
Lactoperoxidase radioiodination to assess surface accessibility.
Immunization of rabbits with soluble extracts.
Indirect membrane immunofluorescence and agglutination assays.

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Main Results:

Ionic shock treatment effectively extracted up to 22 cell surface polypeptides.
Most extracted polypeptides were accessible to radioiodination, indicating surface exposure.
Rabbit antisera against extracts showed reactivity with Raji cell surface.

Conclusions:

Ionic shock treatment is a selective and efficient method for Raji cell surface component extraction.
The extracted polypeptides are surface-localized and immunogenic.
This method facilitates the study of cell surface antigens.