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Effect of degS-degU mutations on the expression of sigD, encoding an alternative sigma factor, and autolysin operon
T Tokunaga1, M H Rashid, A Kuroda
1Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
Journal of Bacteriology
|August 1, 1994
Abstract:
Primer extension analysis of transcripts of the Bacillus subtilis autolysin (cwlB) operon indicated that SigD-dependent transcripts from the Pd promoter are missing in the degU32(Hy) and degS200 (Hy) mutants. The degU32(Hy) mutation caused a 99% reduction in the expression of a sigD-lacZ translational fusion gene constructed in the B. subtilis chromosome. The phosphorylated form of the DegU protein seems to be a regulator for expression of the sigD gene.