Related Experiment Video
Updated: Jul 8, 2026

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
Published on: October 2, 2017
The DNA binding specificity of Ultrabithorax is modulated by cooperative interactions with extradenticle, another
S K Chan1, L Jaffe, M Capovilla
1Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032.
Abstract:
The Ultrabithorax (Ubx) and Antennapedia (Antp) genes of Drosophila encode homeodomain proteins that have very similar DNA binding specificities in vitro but specify the development of different segmental patterns in vivo. We describe cooperative interactions between Ubx protein and a divergent homeodomain protein, extradenticle (exd), that selectively increases the affinity of Ubx, but not Antp, for a particular DNA target. We also provide evidence that Ubx and exd bind to neighboring sites on this DNA and interact directly to stabilize the DNA-bound form of Ubx. Thus, the ability of different homeotic genes to specify distinct segmental patterns may depend on cooperative interactions with proteins such as exd that selectively modulate their otherwise similar DNA binding specificities.
More Related Videos
Related Concept Videos
Nucleosome Remodeling
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Cooperative Binding of Transcription Regulators
Co-activators and Co-repressors
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Single-Strand DNA Binding Proteins
Cooperative Binding of Transcription Regulators

