[Reversible suppression of synaptic function by anti-synaptosome-antibodies (author's transl)]

We report about experiments to isolate Synaptosome-Membranes of the rat cerebellum in order to produce Antibodies directed toward these membranes in rabbits. The raised Antisera were tested in different Complement-fixation-assays. They reacted only with the Synaptosome-Membranes, not with heart, kidney, liver- and spleen-membranes. Different fractions of the isolation-procedure for synatosomal membranes were also checked for their ability to fix Complement. Only Synaptosomes and the synaptosomal membranes gave sufficient Complement fixation, while Mitochondria, soluble brain proteins, synaptic vesicles and braincell nuclei did not. These biochemical and immunological findings are further supported by electrophysiological data, where the transsynaptic component of the parallel fibre-evoked field potential was strongly suppressed by the administration of the immune sera containing antibodies against membranes of synaptosome fractions.