Midline structures and central nervous system coordinates in zebrafish

The embryonic zebrafish provides a relatively simple and accessible experimental system for understanding the underlying Bauplan of a vertebrate central nervous system (CNS) and for uncovering interactions critical for patterning. We show that features of CNS organization in normal, mutant, and developmentally ventralized zebrafish embryos can be explained by a three-axis hypothesis: Specifications occur according to position relative to coordinates along the anterior-posterior, dorsal-ventral, and luminal-pial axes of the neural tube. Midline tissues may be responsible for directly establishing the dorsal-ventral coordinates, but may only be indirectly involved in patterning along the anterior-posterior axis.