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Construction of His6-tagging vectors allowing single-step purification of GroES and other polypeptides produced in
1Lehrstuhl für Genetik, Universität Bayreuth, Germany.
Gene
|September 15, 1994
Abstract:
Two plasmid expression vectors for Bacillus subtilis have been constructed that direct the synthesis of fusion proteins containing a stretch of six histidine residues (His6) at either the N or C terminus. The His6 tag allows the rapid enrichment of proteins by metal chelate affinity chromatography in a denatured or native state. As an example of the general utility of one of these expression vectors, we produced the GroES protein encoded by the groESL operon of B. stearothermophilus.