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Related Experiment Videos

Enteroviruses, endoscopy and infection control: an applied study

P J Hanson1, J Bennett, D J Jeffries

  • 1Department of Medicine, Westminster Hospital, London, UK.

The Journal of Hospital Infection
|May 1, 1994
PubMed
Summary

Disinfection of medical endoscopes requires effective methods against resistant enteroviruses. Alkaline glutaraldehyde rapidly inactivates poliovirus, even when dried, ensuring effective decontamination of medical equipment.

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Medical Device Decontamination

Background:

  • Enteroviruses pose a significant challenge to medical equipment decontamination due to their resistance to standard disinfection protocols.
  • Current decontamination methods primarily rely on bacterial studies, potentially underestimating the required efficacy against more robust viral agents like enteroviruses.

Purpose of the Study:

  • To evaluate the effectiveness of cleaning and disinfection protocols in eliminating enteroviruses from medical endoscopes.
  • To assess the efficacy of 2% alkaline glutaraldehyde in inactivating poliovirus, a representative enterovirus, under various conditions.

Main Methods:

  • Poliovirus was used to contaminate the suction-biopsy channels of five gastroscopes.
  • Endoscopes underwent cleaning with detergent followed by disinfection with 2% alkaline glutaraldehyde.

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  • Viral load was quantified using plaque assays before and after cleaning, and at various disinfection time points.
  • The inactivation of dried, cell-free, and cell-associated poliovirus by glutaraldehyde was also investigated.

    • Main Results:

    • Standard cleaning procedures reduced poliovirus contamination by a mean of 4.6 log10 plaque-forming units (pfu)/mL.
    • Disinfection with 2% alkaline glutaraldehyde rendered the endoscope channels virus-free within 2 minutes.
    • Glutaraldehyde rapidly inactivated dried poliovirus on surfaces within 1 minute, achieving a reduction of over 6 log10 pfu/mL.

    Conclusions:

    • Effective cleaning is crucial for managing high levels of viral contamination on endoscopes.
    • 2% alkaline glutaraldehyde demonstrates rapid and potent antiviral activity against poliovirus, including when dried in organic matter.
    • These findings support the use of glutaraldehyde-based disinfection for ensuring the safety of medical endoscopes against enterovirus contamination.