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Identification and cloning of a second phytase gene (phyB) from Aspergillus niger (ficuum)
K C Ehrlich1, B G Montalbano, E J Mullaney
1Southern Regional Research Center, United States Department of Agriculture, New Orleans, LA 70179.
Biochemical and Biophysical Research Communications
|August 31, 1993
Abstract:
An Aspergillus niger (ficuum) genomic DNA lambda EMBL3 library was probed with a 354-bp DNA fragment obtained by polymerase chain reaction of A. niger DNA with oligonucleotides based on partial amino acid sequence of a pH 2.5 optimum acid phosphatase. A clone containing a 1605 bp segment (phyB) encoding the 479 amino acid enzyme was isolated and found to contain four exons. Global alignment revealed 23.5% homology to Aspergillus niger phytase (PhyA); four regions of extensive homology were identified. Some of these regions may contain catalytic sites for phosphatase function.