Nucleotide sequence of a mouse lamin A cDNA and its deduced amino acid sequence

We have cloned and determined the nucleotide sequence of a mouse lamin A cDNA. The clone contained the C-terminal two-thirds of the lamin A coding sequence and a 3' untranslated sequence with a poly(A) stretch. As has been reported for human lamin A/C cDNAs, a large part of the 5' sequence of our mouse lamin A clone was essentially identical with a previously reported mouse lamin C cDNA sequence, and the deduced C-terminal amino acid sequence shared strong homology with the human lamin A sequence. A putative deduced amino acid sequence for mouse lamin A, which was derived from our sequence and the published lamin C sequence, was 665 amino acids long. The degree of overall homology to the human sequence was more than 95%, and relatively more variation was scattered in the C-terminal lamin A-specific region.