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Cloning, sequence and structural features of a lipase from the antarctic facultative psychrophile Psychrobacter
J L Arpigny1, G Feller, C Gerday
1Laboratoire de Biochimie, Université de Liège-Sart Tilman, Belgium.
Biochimica Et Biophysica Acta
|January 23, 1993
Abstract:
A lipase gene (lip1) from the facultative psychrophilic strain Psychrobacter immobilis B10 has been cloned and sequenced. The deduced preprotein sequence is composed of 317 amino acids with a predicted M(r) of 35,288. A primary structure alignment of lipases including lip1 shows conserved elements for which a structural role is proposed in the light of recent crystallographic studies. The analysis of the psychrophilic enzyme sequence suggests characteristics in relation with the adaptation to cold.