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Nucleotide sequence of the cDNA encoding mouse thyroid peroxidase

T Kotani¹, K Umeki, I Yamamoto

¹Department of Laboratory Medicine, Miyazaki Medical College, Japan.

|January 30, 1993

Summary

Researchers determined the mouse thyroid peroxidase (TPO) cDNA sequence, revealing a 3281 nucleotide sequence encoding a 914 amino acid protein. This finding offers insights into TPO

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Area of Science:

Molecular Biology
Genomics
Biochemistry

Background:

Thyroid peroxidase (TPO) is crucial for thyroid hormone synthesis.
Understanding TPO's genetic and protein structure is vital for research.

Purpose of the Study:

To determine the nucleotide sequence of mouse thyroid peroxidase (TPO) cDNA.
To analyze the homology of mouse TPO with other species.

Main Methods:

cDNA sequencing
Sequence alignment analysis

Main Results:

The mouse TPO cDNA is 3281 nucleotides long, encoding a 914 amino acid protein.
Mouse TPO coding sequence shows high homology with rat TPO (93.5%) and moderate homology with human (75.3%) and porcine (70.8%) TPO.

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Key amino acid sequences, including the active site histidine residue, are conserved across species.

Conclusions:

The determined mouse TPO cDNA sequence provides a valuable resource for comparative studies.
Conserved regions suggest functional importance of specific amino acid residues in TPO activity.