Related Experiment Video

Updated: Aug 19, 2026

Double Whole Mount in situ Hybridization of Early Chick Embryos
15:42

Double Whole Mount in situ Hybridization of Early Chick Embryos

Published on: October 27, 2008

Cloning and sequencing of a chick embryo cDNA encoding the 20-kDa hypusine-containing protein, eIF-5A

M S Rinaudo1, Y A Joe, M H Park

  • 1Laboratory of Cellular Development and Oncology, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892.

Gene
|December 31, 1993
PubMed

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RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...