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Human bradykinin B2 receptor: nucleotide sequence analysis and assignment to chromosome 14
S J Powell1, G Slynn, C Thomas
1Biotechnology Department, ICI Pharmaceuticals, Macclesfield, Cheshire, United Kingdom.
Genomics
|February 1, 1993
Summary
Researchers isolated the human bradykinin B2 receptor gene, crucial for cellular responses. This discovery advances understanding of bradykinin signaling pathways and potential therapeutic targets.
Area of Science:
- Molecular Biology
- Genetics
- Pharmacology
Background:
- The bradykinin B2 receptor (B2R) plays a key role in physiological processes.
- Understanding the human B2R gene is essential for studying its function and regulation.
Purpose of the Study:
- To isolate and characterize the functional cDNA clones for the human bradykinin B2 receptor.
- To determine the gene's structure, sequence, and chromosomal location.
Main Methods:
- Polymerase chain reaction (PCR)-based method using uterus RNA.
- Screening a human cosmid library with a rat B2R probe.
- In vitro transcription and Xenopus oocyte expression system.
Main Results:
- Isolated overlapping clones encoding the entire human B2R protein (364 amino acids).
- Human B2R cDNA shows 81% homology to rat B2R cDNA.
- Functional expression in Xenopus oocytes confirmed by bradykinin-induced membrane currents, blocked by HOE140.
- Identified an intronless genomic clone with promoter and polyadenylation signals.
- Mapped the human B2R gene to chromosome 14.
Conclusions:
- Successfully isolated and characterized the human bradykinin B2 receptor.
- The gene structure and functional expression provide a basis for further research.
- Chromosomal mapping aids in understanding genetic associations and potential disease links.