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[Quantification of DNA using image analysis on paraffin sections]
M Lorenzato1, A Lallemand, B Visseaux-Coletto
1Laboratoire Pol Bouin, CHRU Maison Blanche, Reims.
Annales De Pathologie
|January 1, 1994
Abstract:
With the new softwares that are available, it is now possible to measure the cellular DNA content on Feulgen stained paraffin sections, without enzymatic dissociation of the samples. The main advantage of this technique is to preserve the cellular histological context and to select the right cells that have to be analyzed. The application is of course to obtain information about cancer prognosis, but it can also be very useful in non tumoral pathology. The DNA measurement on paraffin sections can be applied to the diagnosis of triploidy or tetraploïdy in spontaneous abortions without any karyotype.