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Glutamate receptor subtype expression in human postmortem brain

C R Breese1, S S Leonard

  • 1Department of Pharmacology, University of Colorado Health Sciences Center, Denver 80262.

Journal of Molecular Neuroscience : MN
|January 1, 1993
PubMed
Summary
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Antibodies successfully identified glutamate receptor subunits in human brain tissue. This Western blot method allows studying receptor changes in diseases using postmortem samples.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biochemistry

Background:

  • Glutamatergic receptors are crucial for neurotransmission.
  • Characterizing these receptors in human brain is vital for understanding neurological disorders.
  • Postmortem human brain tissue offers a valuable resource for such studies.

Purpose of the Study:

  • To characterize glutamatergic receptors in human postmortem brain tissue.
  • To validate the use of antibodies against glutamate receptor subunits as probes.
  • To investigate the expression levels of AMPA/kainate and kainate receptor subunits in different human brain regions.

Main Methods:

  • Utilized antibodies against rat glutamate receptor subunits (GluR1-3, GluR5).
  • Fractionated crude membranes from rat, monkey, and human postmortem brain tissue via SDS-PAGE.

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  • Detected specific protein bands using Western blotting on nitrocellulose membranes.

    • Main Results:

    • Successfully detected specific glutamate receptor subunits (GluR1-3 at ~105 kDa, GluR5 at ~115 kDa) in human, monkey, and rat brain tissue.
    • Observed varying expression levels of AMPA/kainate and kainate receptor subunits across different human brain regions, with highest expression in hippocampus, temporal cortex, cortex, and cerebellum.
    • Indicated that these glutamate receptor subtypes are glycosylated and showed heterogeneity in expression compared to NCAM.

    Conclusions:

    • Developed and validated a Western blotting technique using antibodies to detect specific glutamate receptor subunits in human postmortem brain.
    • Demonstrated differential expression patterns of AMPA/kainate and kainate receptor subunits in the human brain.
    • This methodology enables the examination of glutamatergic receptor protein alterations in human diseases using postmortem tissue.