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Related Experiment Videos

Proliferating normal bone marrow cells do stain for Ki-67 antigen

G Cattoretti1, A Orazi, J Gerdes

  • 1Department of Pathology, Istituto Nazionale Tumori, Milano, Italy.

British Journal of Haematology
|December 1, 1993
PubMed
Summary

Normal human bone marrow cells show significant MIB 1 antibody staining, a Ki-67 equivalent. This finding contrasts with previous studies and offers new insights into bone marrow cell proliferation markers.

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Area of Science:

  • Hematology
  • Immunohistochemistry
  • Cell Biology

Background:

  • Previous studies reported low proliferation-associated antigen scores in normal human bone marrow.
  • The Ki-67 antibody has been used to assess cell proliferation.

Purpose of the Study:

  • To investigate normal human bone marrow cell proliferation using a new antibody.
  • To re-evaluate proliferation markers in bone marrow using MIB 1 and formalin fixation.

Main Methods:

  • Used MIB 1 antibody, a Ki-67 equivalent.
  • Employed formalin fixation for bone marrow smears and trephine biopsies.
  • Analyzed staining percentages in normal bone marrow cells.

Main Results:

  • Reported 52.8% +/- 9.2 SD of normal bone marrow (BM) cells stained positive for MIB 1.

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  • Observed staining in late forms of maturing blood cells (metamyelocytes, megakaryocytes) in cytokine-treated marrows.

    • Conclusions:

    • Normal human bone marrow exhibits a higher proliferation rate than previously suggested by Ki-67 studies.
    • MIB 1 antibody with formalin fixation provides a more sensitive assessment of bone marrow cell proliferation.