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Gene amplification in non-Hodgkin's lymphoma
D Ben-Yehuda1, J Houldsworth, N Z Parsa
1Laboratory of Cancer Genetics, Memorial Sloan-Kettering Cancer Center, New York, N.Y.
British Journal of Haematology
|April 1, 1994
Summary
Gene amplification, indicated by homogeneously staining regions (HSRs), was observed in diffuse large cell lymphoma (DLCL). Further analysis suggests the amplification of novel genes in these non-Hodgkin
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Gene amplification is a known mechanism in cancer development.
- Homogeneously staining regions (HSRs) are cytological indicators of gene amplification.
- Non-Hodgkin's lymphoma (NHL) is a diverse group of lymphoid malignancies.
Purpose of the Study:
- To investigate gene amplification in large cell diffuse lymphoma (LC-DL).
- To identify candidate amplified genes in NHL tumors exhibiting HSRs.
- To determine if known protooncogenes or drug resistance genes are amplified.
Main Methods:
- Southern blot analysis of tumor DNA.
- Use of probes for 23 protooncogenes and the multidrug resistance gene (PGY1).
- DNA in-gel renaturation assay to confirm DNA amplification.
Main Results:
- HSRs were found in nine of 426 NHL tumors, specifically in LC-DL.
- Two cases showed a two-fold amplification of the BCL2 gene.
- DNA in-gel renaturation confirmed amplified DNA fragments in four of seven specimens, suggesting novel gene amplification.
Conclusions:
- The study identified gene amplification in a significant subset of LC-DL.
- Amplification of known protooncogenes or PGY1 was not consistently found.
- Results indicate the potential amplification of novel genes in these lymphoma subtypes.