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Cloning of human and rat p69 cDNA, a candidate autoimmune target in type 1 diabetes
I Miyazaki1, R Gaedigk, M F Hui
1Hospital for Sick Children, Department of Paediatrics, University of Toronto, Ontario, Canada.
Biochimica Et Biophysica Acta
|October 21, 1994
Abstract:
Triggering of autoimmunity in insulin-dependent diabetes was linked to dietary bovine serum albumin (BSA). Anti-BSA antibodies from diabetes-prone rats precipitate a protein, p69, from islet cell lysates. We have used these antibodies to identify rat p69 cDNAs. Human p69 cDNA was identified by crosshybridization. The p69 coding regions show 87% nucleotide and 89% amino acid homology. Recombinant p69 is recognized by autoantibody and T cells from diabetic children.