Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Initial characterization of equine inhibin

K H Moore1, B S Dunbar, G R Bousfield

  • 1Department of Biochemistry and Molecular Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030.

Biology of Reproduction
|July 1, 1994
PubMed
Summary

Equine inhibin, a reproductive hormone, is present in follicular fluid, with most activity found in larger molecular forms. This study characterizes equine inhibin subunits and their molecular weights, revealing similarities to porcine inhibin.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bacterial colonization of bladder urothelial cells in women with refractory Detrusor Overactivity: the effects of antibiotic therapy.

Pathogens and disease·2021
Same author

The association between nocturia, hormonal symptoms and bladder parameters in women: an observational study.

BJOG : an international journal of obstetrics and gynaecology·2021
Same author

Laparoscopic sacrocolpopexy: how low does the mesh go?

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2016
Same author

Omega 3 and omega 6 fatty acids in human and animal health: an African perspective.

Molecular and cellular endocrinology·2014
Same author

ATP during early bladder stretch is important for urgency in detrusor overactivity patients.

BioMed research international·2014
Same author

'Urinary incontinence' post-radiotherapy: a diagnostic conundrum.

Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology·2010

Area of Science:

  • Reproductive Endocrinology
  • Molecular Endocrinology
  • Comparative Physiology

Background:

  • Inhibin characterization is limited in horses compared to other mammals.
  • Understanding equine inhibin is crucial for reproductive studies.

Purpose of the Study:

  • To characterize the size heterogeneity of equine inhibin alpha- and beta-subunits.
  • To analyze the molecular distribution of inhibin activity in equine follicular fluid.

Main Methods:

  • Western blot analysis of equine follicular fluid.
  • Size exclusion chromatography (S-200 column).
  • One-dimensional (1D) and two-dimensional (2D) SDS-PAGE, including isoelectric focusing (IEF).

Main Results:

Related Experiment Videos

  • Equine inhibin activity predominantly exists in higher molecular weight forms.
  • Western blot detected alpha-subunit bands at 40, 56, 80, and 90 kDa; beta-subunit bands at 56 and 90 kDa.
  • Lower molecular weight inhibin (32 kDa) and its subunits (alpha at 18 kDa, beta at 14 kDa) were identified, showing similarities to porcine inhibin.

    • Conclusions:

    • Inhibin is abundant in equine follicular fluid, with a greater proportion in higher molecular weight forms than in porcine inhibin.
    • The lower molecular weight equine inhibin shares size and 2D-PAGE patterns with porcine inhibin.
    • This research provides detailed characterization of equine inhibin subunit composition and molecular forms.