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Evaluation of chemiluminescent detection of bromodeoxyuridine-labeled DNA
L López-Canovas1, A López-Brauet, M D Del Castillo-Bilbao
1Department of Molecular Biology, Centro Nacional de Investigaciones Científicas, Havana, Cuba.
Archives of Medical Research
|January 1, 1994
Abstract:
Colorimetric immunoenzymatic procedures using monoclonal antibodies to detect 5-bromo-2-deoxy-uridine-DNA in hybridization experiments are not sensitive enough. We have developed a chemiluminescent immunodot assay to improve the sensitivity of the immunoenzymatic procedure, and attained a suitable detection level for single-stranded DNA. This new chemiluminescent immunodot assay has also been used to detect our recently synthesized cytidine analogue by means of its corresponding monoclonal antibody.