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A genetic map of the mouse with 4,006 simple sequence length polymorphisms
W F Dietrich1, J C Miller, R G Steen
1Whitehead Institute/MIT Center for Genome Research, Cambridge, Massachusetts 02142.
Nature Genetics
|June 1, 1994
Summary
Researchers created a detailed genetic map of the mouse genome using 4,006 simple sequence length polymorphisms (SSLPs). This high-resolution map significantly advances mouse genetic research and disease studies.
Area of Science:
- Genomics
- Mouse Genetics
Background:
- Accurate genetic maps are crucial for understanding genome organization and function.
- Previous mouse genetic maps had limitations in marker density and coverage.
Purpose of the Study:
- To construct a high-density genetic map of the mouse genome.
- To provide a valuable resource for positional cloning and comparative genomics in mice.
Main Methods:
- Development and genotyping of 4,006 simple sequence length polymorphisms (SSLPs).
- Integration of SSLP data to create a comprehensive genetic map.
Main Results:
- A genetic map with an average marker spacing of 0.35 centiMorgans (cM) was generated.
- Approximately 90% of the mouse genome is within 1.1 cM of a marker.
- Markers exhibited a 50% polymorphism rate in laboratory mouse crosses, with some underrepresentation on the X chromosome.
Conclusions:
- The constructed map represents a significant advancement in mouse genome mapping.
- This resource facilitates gene discovery and functional studies in mouse models.
- Further development aims to reach a 6,000 SSLP marker density.